Beclin-1 (Beclin 1 (Coiled-coil Moesin-like BCL2-interacting Protein), Beclin1, BECN1, APG6, ATG6, ATG6 Autophagy-related 6 Homolog, Coiled-coil Myosin-like Bcl-2-Interacting Protein, GT197, Protein GT197, VPS30) (HRP)

Size

£685.00

SKU:: B0981-23T
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Internal synthetic peptide to human Beclin 1, containing residues within 1-100. Cellular Localization: Cell Membrane, Cytoplasmic and Golgi Apparatus. Expressed in dendrites and cell bodies of cerebellar Purkinje cells.
Format:: Supplied as a liquid in PBS. Labeled with HRP. No preservative added.
Extra Details:: Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumor suppressor and antiviral function. Autophagy, a process of bulk protein degradation through an autophagosomic-lysosomal pathway, is important for differentiation, survival during nutrient deprivation, and normal growth control, and is often defective in tumor cells. Beclin 1 was originally isolated in a yeast two hybrid screen to identify Bcl-2-binding partners and maps to a tumor susceptibility locus on human chromosome 17q21 that is frequently monoallelically deleted in human breast, ovarian and prostate cancer. Beclin 1 encodes an evolutionarily conserved 60 kDa coiled coil protein that is expressed in human muscle, epithelial cells and neurons. In gene transfer studies, beclin 1 promotes nutrient deprivation-induced autophagy, inhibits mammary tumorigenesis, and inhibits viral replication. Expression of the Beclin 1 protein is frequently decreased in malignant breast epithelial cells. Based upon these observations, it is speculated that beclin 1 may work through induction of autophagy to negatively regulate tumorigenesis and to control viral infections. Beclin 1 may also play a role in other biological processes in which autophagy is important such as cell differentiation and nutritional stress responses. Applications: Suitable for use in Western Blot, Immunofluorescence, Immunohistochemistry (paraffin). Other applications not tested. Recommended Dilution: Western Blot: 1:2000-1:5000, a band is seen at ~52 kD Immunofluorescence: mouse tissue Optimal dilution determined by the researcher. Storage and Stability: Store at 2-8°C. Do Not Freeze! Aliquots are stable for at least 3 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Shipping Conditions:: Blue Ice