BD3 (beta Defensin-3)

Size

£793.00

SKU:: B0902
Application:: WB
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: A synthetic peptide consisting of aa 23-33 (GIINTLQKYYC) of the human beta-defensin-3 protein
Format:: Supplied as a liquid in PBS, 0.02% sodium azide.
Extra Details:: Beta Defensin-3 is a novel 5-9kD nonhemolytic antimicrobial protein peptide which may be important in the innate epithelial defense of infections by various microorganisms seen in skin and lung (such as cystic fibrosis). Applications: Suitable for use Western Blot. Other applications not tested. Recommended Dilution: Western Blot: Tricine-SDS-PAGE gels: 1:500-1:1000. Migrates as a 5-6kD band. Tricine-SDS-urea PAGE gels: Native Beta Defensin 3 migrates as a 9kD band. The larger size is because of the reduction of the three cysteine bridges present in the peptide protein. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.