B0901-03B

BD2 (beta Defensin-2, beta 2 Defensin, Defensin beta 2, BD 2, BD-2, DEFB2, DEFB-2, HBD 2, HBD2, HBD-2, DEFB102, Skin Antimicrobial Peptide 1, SAP1)

Size

£735.00

SKU:: B0901-03B
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Recombinant human Defensin beta 2, expressed in E. coli.
Format:: Supplied as a lyophilized powder from PBS, pH 7.4. No preservative added. Reconstitute with sterile ddH2O to 0.1-1mg/ml.
Extra Details:: Expression of BD-2 is induced during inflammation in response to bacterial products and cytokines, and is initially expressed in a precursor form, which is cleaved to release the C-terminal active portion of the protein. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Sandwich ELISA: 0.5-2ug/ml using 100ul/well. In conjunction with B0901-03B1 as detection antibdy allows the detection of at least 2000-4000 pg/ml of recombinant human BD-2 Western Blot: 0.1-0.2ug/ml. The detection limit for recombinant human BD2 is 1.5-3ng/lane under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.