Bcl-XL

Size

£655.00

SKU:: B0807-19D
Purity:: Purified by Ion Exchange Chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Peptide corresponding to 14 amino acids near the C-terminus of human Bcl-xL. Species Sequence Homology: porcine, mouse and rat
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.
Extra Details:: Recognizes the long isoform of human Bcl-2-like protein 1, otherwise known as Bcl-xL, located on the mitochondrial membrane. The Bcl-xL isoform (the longer isoform) is a potent inhibitor of apoptosis. It appears to regulate cell death by binding to and blocking the voltage-dependent anion channel, thereby preventing the release of the caspase activator cytochrome c. High expression of Bcl-xL in tumors correlates to advanced disease and a poor prognosis. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml, detects a band of ~27kD in A549 cell lysate Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.