B0807-15F

Bcl-XL (FITC)

Size

£576.00

SKU:: B0807-15F
Purity:: Purified
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant human Bcl-xS
Clone:: 7B2.5
Format:: Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Extra Details:: Apoptosis, or programmed cell death, is a well-documented phenomenon in many cellular systems.1 It plays a key role in tissue and organ development as well as in adult tissues during cell turnover. Apoptosis can be induced by a variety of internal and external stimuli including growth factor deprivation, cytokine treatment, antigen-receptor engagement, cell-cell interactions, irradiation and glucocorticoid treatment.2 Bcl-2 and one of its homologues, Bcl-xL, protect cells from apoptosis3,4, while other homologues of Bcl-2 such as Bax, Bad and Bak have been shown to enhance apoptosis.5-8 Bcl-xL has been shown to block apoptosis which is induced by a variety of stimuli and, under certain conditions, offers greater protection against apoptosis than Bcl-2.9-13 In contrast, Bad and Bax inhibit the protective functions of Bcl-xL and Bcl-2, respectively. Although heterodimerization between Bcl-xL/Bad and Bcl-2/Bax was originally thought to be essential for the differential anti-apoptotic activity of Bcl-xL and Bcl-2,5,14 other results suggest that the formation of heterodimers may not be necessary for this death-repressing activity.15,16 Applications: Suitable for use in Immunoprecipitation, Flow Cytometry, Immunohistochemistry and Western Blot. Other applications have not been tested. Recommended Dilutions: Flow Cytometry: ≤ 3ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice