BCLX (Bcl-2-like Protein 1, Bcl2-L-1, Apoptosis Regulator Bcl-X, BCL2L, BCL2L1)

Size

£512.00

SKU:: B0807-14Y
Purity:: Purified by immunoaffinity chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to a sequence at the middle region of human Bcl-x/s (different to the related rat sequence by a single aa.)
Format:: Supplied as a lyophilized powder from PBS, pH 7.4 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Extra Details:: Bcl-x, a member of the Bcl-2 protein family, inhibits cell death, or apoptosis. Bcl-x is expressed as two isomeric forms, Bcl-xL and Bcl-xS, and is typically present in the cytosol in association with the mitochondrial membrane. Bcl-xL forms heterodimers with various proteins, including Bax, Bak and Bcl-2. It has been found that heterodimerization with Bax does not seem to be required for anti-apoptotic activity. Since Bcl-xL can form an ion channel in synthetic lipid membranes, there is a strong possibility that this property plays a role in heterodimerization-independent cell survival. The Bcl-X(S) isoform promotes apoptosis. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.1-1ug/ml Immunohistochemistry (Paraffin): 0.5-2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice