B0807-14X-APC
Bcl-X (Bcl2 Like 1 Protein, Apoptosis Regulator Bcl-X, Bcl2-L-1, Bcl-2-like Protein 1, BCL2L1, BCLX) (BSA & Azide Free) (APC)
Size
£931.00
- SKU:
- B0807-14X-APC
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to residues 3-14 (Cys-QSNRELVVDFLS) of human Bcl-X protein. Cellular Localization: Cytoplasmic and cell/nuclear membrane.
- Clone:
- 2H12
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
- Extra Details:
- Bcl-X has 2 isoforms, Bcl-Xl (long) a 241 amino acid protein and Bcl-Xs (short) a 178 amino acid protein lacking a 63 amino acid domain that is well conserved among members of the Bcl-2 family. Applications: Suitable for use in Flow Cytometry, Immunofluorescence, Immunoprecipiation and Immunohistochemistry. Not suitable for use in Western Blot. Other applications have not been tested. Recommended Dilution: Immunoprecipitation (Use Protein A): 2ug/mg protein lysate Immunohistochemistry (formalin/paraffin): 2-4ug/ml for 30min at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20min followed by cooling at RT for 20min. Optimal dilutions to be determined by the researcher. Positive Control: Hodgkin’s lymphoma Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice