B0807-06K

Bcl-2 (B Cell Leukemia 2)

Size

£406.00

SKU:: B0807-06K
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant corresponding to aa1-211 from human Bcl-2 expressed in E. coli.
Clone:: 11H1
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Extra Details:: Bcl-2 (B-cell lymphoma protein 2 alpha) is an anti-apoptotic protein located primarily in the outer mitochondrial membrane. It blocks the apoptotic death of some cells such as lymphocytes. BCL-2 is thought to regulate cell death by controlling the mitochondrial membrane permeability during apoptosis. Bcl-2 protein has anti-apoptotic functions through inhibiting caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Applications: Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:250 - 500 Starting dilution: 1:500 Optimal dilutions to be determined by the researcher. Hybridoma: F0 myeloma cells with spleen cells from BALB/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.