B0807-06F1

Bcl-2 (B Cell Leukemia 2) (BSA and Azide Free) (Biotin)

Size

£588.00

SKU:: B0807-06F1
Purity:: Purified by Protein A affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide corresponding to aa41-54 of human Bcl-2.
Clone:: 7H161
Format:: Supplied as a liquid in PBS. BSA and Azide-free. Labeled with Biotin.
Extra Details:: The human bcl-2 gene family plays a central role in lymphoid system cell survival. Continuous expression of bcl-2 protein is critical for preventing apoptosis of mature peripheral B and T cells. Applications: Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Fixed, paraffin sections Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Cultured Nalm-6 human tumor cells were fixed for 30 min with 2% buffered formaldehyde after which they were washed two times. 2. Subsequent reagent incubations and washes were done using a buffer containing 0.3% Saponin to permeabolize cells. 3. Five x 10e5 cells per tube were incubated 45 minutes on ice with 80ul of B0807-06F at 5ug/ml. 4. Cells were then washed twice and incubated with S7973-88P Streptavidin (PE). 5. Cells were washed three times, fixed and analyzed by FACS. 6. Cells stained with a mean shift of 0.39 log10 fluorescent units when compared to a Mouse IgG1 negative control (Biotin) at a similar concentration. 7. This shift was blocked when permeabalized cells were preincubated 10 minutes with 20ul of 0.5mg/ml B0807-06E. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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