B0807-06F
Bcl-2 (B Cell Leukemia 2) (Biotin)
Size
£607.00
- SKU:
- B0807-06F
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to aa41-54 of human Bcl-2.
- Clone:
- 7H161
- Format:
- Supplied as a liquid in 50mM sodium phosphate pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Biotin.
- Extra Details:
- The human bcl-2 gene family plays a central role in lymphoid system cell survival. Continuous expression of bcl-2 protein is critical for preventing apoptosis of mature peripheral B and T cells. Applications: Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 5ug/ml; 80ul labels 5x10e5 Nalm-6 tumor cells Immunohistochemistry: Fixed, paraffin sections Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Cultured Nalm-6 human tumor cells were fixed for 30 min with 2% buffered formaldehyde after which they were washed two times. 2. Subsequent reagent incubations and washes were done using a buffer containing 0.3% Saponin to permeabolize cells. 3. Five x 10e5 cells per tube were incubated 45 minutes on ice with 80ul of B0807-06F at 5ug/ml. 4. Cells were then washed twice and incubated with S7973-88P Streptavidin (PE). 5. Cells were washed three times, fixed and analyzed by FACS. 6. Cells stained with a mean shift of 0.39 log10 fluorescent units when compared to a Mouse IgG1 negative control (Biotin) at a similar concentration. 7. This shift was blocked when permeabalized cells were preincubated 10 minutes with 20ul of 0.5mg/ml B0807-06E. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice