B0807-05R

Bcl 2 (B Cell Leukemia 2)

Size

£416.00

SKU:: B0807-05R
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: KLH-conjugated peptide corresponding to 61-76 amino acid sequence of murine Bcl-2
Clone:: 10C2
Format:: Supplied as a liquid in 100mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.
Extra Details:: Apoptosis, or programmed cell death, is a well-documented phenomenon in many cellular systems.2 It plays a key role in tissue and organ development as well as in adult tissues during cell turnover. Apoptosis can be induced by a variety of internal and external stimuli including growth factor deprivation, cytokine treatment, antigen-receptor engagement, cell-cell interactions, irradiation and glucocorticoid treatment.3 Bcl-2 is a widely studied protein that has been shown to be a potent inhibitor of programmed cell death. It has been localized to the outer mitochondrial membrane, perinuclear membrane, and endoplasmic reticulum. Bcl-2 is expressed in memory and resting, or other long-lived lymphoid cells, follicular mantle cells, medullary thymocytes, and lymphomas. Germical center cells and cortical thymocytes are negative for Bcl-2. Upregulation of Bcl-2 prevents or delays apoptosis induced by a variety of stimuli, including growth factor deprivation, gamma-irradiation, glucocorticoids, and chemotherapeutic agents. During lymphoid development, expression of the Bcl-2 protein appears to be regulated in a stage-specific manner, and is thought to be a survival signal for positive selection. Applications: • Western Blot • Enzyme-Linked-Immunosorbent-Assay (ELISA) Recommended Dilution: Western Blot: ≤ 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice