B0807-05H

Bcl 2 (B Cell Leukemia 2)

Size

£620.00

SKU:: B0807-05H
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide corresponding to aa41-54 of human Bcl2.
Clone:: 100
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Extra Details:: Bcl-2 is a conserved anti-apoptotic protein that plays important roles in normal immunity. It forms homodimers or heterodimers with other Bcl-2 family members. This protein regulates apoptosis through controlling mitochondrial fusion and fission. Phosphorylation of Bcl-2 has been shown to enhance activity to allow response to extracellular growth-factor-mediated signals. Bcl-2 is also regulated by IRES, miR-15a, miR-16-1 and RNA-BP nucleolin. Chromosome 18q21.3 translocation and overexpression of Bcl-2 is frequently observed in follicular lymphomas and some diffuse large B-cell lymphomas. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-2ug/ml Immunohistochemistry (FFPE): 5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.