B0807-05E
Bcl 2 (B Cell Leukemia 2)
Size
£655.00
- SKU:
- B0807-05E
- Purity:
- Purified by Ion Exchange chromatography.
- Storage Conditions:
- 4°C (-20°C Glycerol)
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to 15 amino acids near the N-terminus of human Bcl-2 (GenBank accession no. AAH27258)
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.
- Extra Details:
- Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer (reviewed in 1). Bcl-2 is the founding member of a family of over 20 proteins that are critical regulators of apoptosis (reviewed in 2 and 3). These can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Bcl-2 is an inner mitochondrial membrane protein that inhibits apoptosis (4). It is thought to act by interacting with pro-apoptotic Bcl-2 family members such as Bak and Bad (5 for review). Overexpression of Bcl-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer (4,6). Applications: Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Immunohistochemistry: 2ug/ml; Stains human kidney section. Western Blot: 1-2ug/ml Optimal dilutions to be determined by the researcher. Control Peptide: B0807-05E1: Bcl 2 (Control Peptide) (B Cell Leukemia 2) Positive Control: WB: Daudi cell lysate Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice