B0807-02F2
Bcl-2 (Apoptosis Regulator Bcl-2, BCL2)
Size
£512.00
- SKU:
- B0807-02F2
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to residues 41-54 of the bcl-2 protein (thyroglobulin).
- Clone:
- 10K110
- Format:
- Supplied as a lyophilized powder from 1.2% sodium acetate, 2mg BSA, 0.01mg sodium azide. Reconstitute with 1ml sterile PBS.
- Extra Details:
- Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml detects Bcl-2 in cells and tissues Immunohistochemistry (FFPE): 0.4-1ug/ml Immunohistochemistry (Frozen): 0.4-1ug/ml in formalin- acetone fixed tissues Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice