B0401
B Cells (FITC)
Size
£800.00
- SKU:
- B0401
- Application:
- Flow Cytometry
- Purity:
- Purified ascites. Tested by electrophoresis and Flow Cytometry.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- The human B-lymphoblastoid cell line, HRIK.
- Clone:
- 2Q385 (FMC-7)
- Format:
- Supplied as a liquid in 0.1M Tris-HCl, 0.5M sodium chloride, 10mM glycine, pH 8.0, 0.2% BSA, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
- Extra Details:
- Studies on normal lymphocytes, leukemic cells and cell lines indicate it is a marker for a limited segment of the B cell maturation pathway(2,3). This glycoprotein is expressed in varying degrees on normal circulating B-cells, chronic B-cell leukemia (B-CLL), prolymphocytic leukemia (PLL) and other B cell neoplasias (4–1)2. It labels the same population as CD22 antibodies but reacts with a different antigen(5). Cell reactivity: Stains peripheral blood B lymphocytes and tonsil B lymphocytes. (1). This antibody can be used for the differentiation of PLL from B-CLL. It is also useful in confirmation of the diagnosis of other disorders such as HCL, HCL-V and SLVL. Useful for the detection of residual disease in patients undergoing treatment for these disorders. Applications: Suitable for use in Flow Cytometry. Other applications have not been tested. Recommended Dilution: Flow Cytometry: Neat. Use 10ul per 100ul of whole blood, or 1x10e6 peripheral blood mononuclear cells (PBMC) in 100ul buffer. Optimal dilution to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice