Basic Hair Keratin, K83 (KRT83, Keratin 83, HB3, Hb-3, K2.10, K83, Keratin, type II cuticular Hb3, Keratin-83, KRTHB3, MGC141663, Type II hair keratin Hb3) (Not for Export EU)

Size

£960.00

SKU:: B0130-23
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Guinea Pig
Immunogen:: Synthetic peptide corresponding to CGFNSIGCGFRPGNF of human basic hair (trichocytic) keratin K83 conjugated to KLH. (Formally designated keratin hHb3).
Format:: Supplied as a liquid in 0.5% BSA, 0.09% sodium azide.
Extra Details:: Applications: Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Immunohistochemistry (Frozen): 1:200; Specifically stains human hair keratin KB3 expressed starting from the middle cortex. For enhancement of cortex staining perincubate fixed sections with PBS, 0.1 Triton X-100 for 1-5 minutes prior to first incubation. Incubate 1 hour at RT. Western Blot (ECL): 1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.