B0099

PTP-Bas, Recombinant, Human, aa2091-2490, GST-Tag (PTPL1, FAP-1, PTPN13)

Size

£951.00

SKU:: B0099
Molecular Weight:: 71.8
Purity:: ≥90% by SDS-PAGE.
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 75mM sodium chloride, 0.05% Tween 20, 2mM EDTA, 1mM DTT, 10mM glutathione, 50% glycerol.
Extra Details:: Recombinant PTP-Bas, catalytic domain, aa2091-2490 (catalytic domain), with N-terminal GST tag, expressed in an E. coli. Specific Activity: As Reported Application: Suitable for use in the study of enzyme kinetics, regulation and to dephosphorylate target substrates. Enzyme Assay Buffer and Substrate: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 50mM pNPP. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.