B0076-12
BAP1 (BRCA1 Associated Protein 1, Ubiquitin carboxyl-terminal hydrolase BAP1, EC 3.4.19.12, BRCA1-associated protein 1, Cerebral protein 6, BAP-1)
Size
£594.00
- SKU:
- B0076-12
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified full-length human BAP1 expressed in E. coli. Epitope: Within the UCH domain of BAP1. Cellular Localization: Nuclear
- Clone:
- 8B66 (1G8)
- Format:
- Supplied as a liquid in Tris-glycine, 150mM sodium chloride, 0.05% sodium azide.
- Extra Details:
- Mutations within the BRCA1 gene, localized to chromosome 17q, are believed to account for approximately 45% of families with increased incidence of both early-onset breast cancer and ovarian cancer. The BRCA1 gene is expressed in numerous tissues, including breast and ovary, and encodes a predicted protein of 1863 amino acids. This protein contains a RING domain near the N-terminus and appears to encode a tumor suppressor. BARD1 (BRCA1-associated RING domain protein 1) and BAP1 (BRCA1-associated protein 1) have both been shown to bind to the N-terminus of BRCA1 and are potential mediators of tumor suppression. BARD1 contains an N-terminal RING domain and three tandem ankyrin repeats. The C-terminus of BARD1 contains a region with sequence homology to BRCA1, termed the BRCT domain. BAP1 is a ubiquitin hydrolase and has been shown to enhance BRCA1-mediated cell growth suppression. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 2ug/ml Optimal dilutions to be determined by the researcher. Positive Control: HeLa whole cell lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice