B0055-10-BIOTIN

Bak, NT (BAK1, BCL2-antagonist/killer 1, BAK, CDN1, BCL2L7, cell death inhibitor 1, apoptosis regulator BAK, Bcl-2 homologous antagonist/killer) (Biotin)

Size

£1,157.00

SKU:: B0055-10-BIOTIN
Purity:: Purified by Protein A affinity chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to amino acid residues 23-38 (SEEQVAQQDTEEVFRSC) of human Bak with a cysteine residue added to the C-terminus for conjugation to KLH.
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Extra Details:: Bak or Bcl2 homologous antagonist is a member of the Bcl2 family of proteins. The Bcl-2 related proteins interact with one another through the formation of homo and heterodimers. The susceptibility of cells to apoptotic stimuli is thought to be controlled by the relative ratios of the different Bcl2 family proteins. Bak has been demonstrated to accelerate the rate of apoptosis in growth factor deprived murine lymphoid, neuronal and fibroblastic cell lines. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. Bak also interacts with the tumor suppressor P53 after exposure to cell stress. Applications: Suitable for use in Western Blot, Immunohistochemistry, immunocytochemistry and Immunoprecipitation. Other applications have not been tested. Recommended Dilution: Immunohistochemistry: Paraffin sections Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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