B0055-01N

Bak (BAK1, BCL2-antagonist/killer 1, CDN1, BCL2L7, Cell death inhibitor 1, Apoptosis Regulator BAK)

Size

£895.00

SKU:: B0055-01N
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Recombinant protein corresponding to Pro20-Asn124 from human BAK, expressed in E. coli
Format:: Supplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: Bak (BAK1, BCL2-antagonist/killer 1, BAK, CDN1, BCL2L7, cell death inhibitor 1, apoptosis regulator BAK, Bcl-2 homologous antagonist/killer) is a 25-30kD member of the BCL-2 family of proteins. It is widely expressed, and participates in the apoptotic cycle. BAK is an outer mitochondrial membrane protein that is inactive as a Zn-dependent homodimer. Upon activation by p53 or tBID, BAK oligomerizes, creating a pore in the mitochondrial membrane and allowing for cytochrome C release. Human BAK is 211 aa in length and contains three BCL-2 homology domains (aa74-88, 117-136 and 169-184), a Zn-binding region (aa160-166) and a C-terminal transmembrane segment (aa188-205). Amino acids 67-94 mediate oligomerization of BAK. There are two potential isoform variants; one shows an alternate start site at Met96, while a second shows a deletion of aa46-66. Over amino acids 20-124, human BAK shares 76% aa identity with mouse BAK. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml Immunohistochemistry: 5-15ug/ml using immersion fixed paraffin-embedded sections of human colon Immunocytochemistry: 5-15ug/ml in immersion fixed HEK293 human embryonic kidney cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice