B0055-01A
Bak (BAK1, BCL2-antagonist/killer 1, BAK, CDN1, BCL2L7, cell death inhibitor 1, apoptosis regulator BAK, Bcl-2 homologous antagonist/killer)
Size
£512.00
- SKU:
- B0055-01A
- Purity:
- Purified by Protein A affinity chromatography
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to aa2-14, ASGQGPGPPRQEC, of human Bak conjugated to KLH.
- Format:
- Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
- Extra Details:
- Bak is a 23kD homolog of the apoptosis inhibitor, Bcl-2 with an apparent molecular weight of 30kD on SDS-PAGE. Bak is a member of the Bcl-2 family of proteins and contains the bcl-2 homology regions (BH-) 1, 2 and 3. Unlike Bcl-2, Bak promotes apoptosis by interacting with and counteracting the anti-cell death effect of Bcl-2. Enforced expression of Bak induces rapid and extensive apoptosis of serum-deprived fibroblasts (1). However, Bak can inhibit cell death in an Epstein-Barr virus transformed cell line (2). Applications: Suitable for use in Western Blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot (ECL): 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry: 1:50 Optimal dilutions to be determined by the researcher. Positive Controls: HeLa Heat Shocked Cell Lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice