B0003-68Y2

BAFF Receptor (BAFFR, BAFF-R, B Cell-activating Factor Receptor, BLyS Receptor 3, BR3, BROMIX, CD268, CVID4, MGC138235, Prolixin, Tumor Necrosis Factor Receptor Superfamily Member 13C, TNFRSF13C) (Biotin)

Size

£639.00

SKU:: B0003-68Y2
Application:: Flow Cytometry
Purity:: Purified by Protein A affinity chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human BAFF Receptor
Clone:: 11K1
Format:: Supplied as liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Biotin.
Extra Details:: Human CD268 (BAFFR, BAFF receptor) is a type I TNF superfamily receptor member #13c. It is highly specific for CD257 (BAFF), which itself does bind to other receptors TACI, and BCMA. CD268 expressed on B cells and its ligation by CD257 (BAFF) regulates maturation. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Wash 5x10e5 cultured human Raji cells per tube and incubated 45 minutes on ice with 80ul of B0003-68Y2 at 10ug/ml. 2. Cells were washed twice and incubated with secondary reagent Streptavidin/R-PE (S7973-88P) after which they were washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 1.1 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. 3. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml B0003-68Y. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice