B0003-68Y

BAFF Receptor (BAFFR, BAFF-R, B Cell-activating Factor Receptor, BLyS Receptor 3, BR3, BROMIX, CD268, CVID4, MGC138235, Prolixin, Tumor Necrosis Factor Receptor Superfamily Member 13C, TNFRSF13C)

Size

£557.00

SKU:: B0003-68Y
Purity:: Purified by Protein A affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human CD268
Clone:: 11K1
Format:: Supplied as liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.5mg/ml gentamicin sulfate.
Extra Details:: Human CD268 (BAFFR, BAFF receptor) is a type I TNF superfamily receptor member #13c. It is highly specific for CD257 (BAFF), which itself does bind to other receptors TACI, and BCMA. CD268 expressed on B cells and its ligation by CD257 (BAFF) regulates maturation (1). Applications: Suitable for use in ELISA and Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5ug/ml; 80ul labels 5x10e5 cultured human Raji cells Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 human Raji cells per tube were washed and incubated 45 minutes on ice with 80ul of B0003-68Y at a concentration of 5ug/ml. 2. Cells were washed twice and incubated with IgG (FITC) goat anti-mouse after which they were washed twice, fixed and analyzed using FACS. 3. Cells stained positive with a mean shift of 0.79 log10 fluorescent units when compared to isotype control Mouse IgG1 antibody at a similar concentration. 4. Antbody binding was partially blocked when reagent was pre-incubated with a 10-fold excess of recombinant CD268-muIg. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice