B0003-47F

Bad, phosphorylated (Ser136) (Bcl2 Antagonist of Cell Death, Bcl-2 Antagonist of Cell Death Protein, BBC2, Bcl-2-binding Component 6, BBC6, BCL-X/Bcl-2 Binding Protein, BCL-X Binding Protein, Bcl-XL/Bcl-2 Associated Death Promoter, Bcl2 Antagonist of Cell Death, BCL2 Associated Agonist of Cell Death, Bcl2 Associated Death Promoter, BCL2 Binding Component 6, BCL2 Binding Protein, Bcl2-like 8 Protein, BCL2L8, Bcl2-L-8, Bcl xL, Bcl-xL, Proapoptotic BH3 Only Protein)

Size

£672.00

SKU:: B0003-47F
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic phosphopeptide corresponding to residues surrounding Ser136 of mouse Bad. Species Sequence Homology: rat
Clone:: 10E8(D25H8)
Format:: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Extra Details:: Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL (1,2). Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136 (2). Phosphorylation at these sites promotes binding of Bad to 14-3-3 proteins to prevent an association between Bad with Bcl-2 and Bcl-xl (2). Akt phosphorylates Bad at Ser136 to promote cell survival (3,4). Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK (5,6) and mitochondria-anchored PKA (7). Phosphorylation of Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL (8-10). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice