Bacillus cereus/Bacillus subtilis, Spores

Size

£256.00

SKU:: B0003-27
Application:: IF
Purity:: Purified by Protein A affinity chromatography (≥95% SDS-PAGE).
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Purified spores of B. cereus (ATCC #11778) + B. subtilis (ATCC #9372)
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. No stabilizing proteins have been added.
Extra Details:: Bacillus cereus is an endemic, soil-dwelling, Gram-positive, rod-shaped, beta hemolytic bacterium. Some strains are harmful to humans and cause foodborne illness, while other strains can be beneficial as probiotics for animals. B. cereus bacteria are facultative anaerobes, and like other members of the genus Bacillus can produce protective endospores. Bacillus subtilis, known as the hay bacillus or grass bacillus, is a Gram-positive, catalase-positive bacterium commonly found in soil. A member of the genus Bacillus, B. subtilis is rod-shaped, and has the ability to form a tough, protective endospore, allowing the organism to tolerate extreme environmental conditions. Unlike several other well-known species, B. subtilis has historically been classified as an obligate aerobe, though recent research has demonstrated that this is not strictly correct. Applications: Suitable for use in Immunofluorescence, Fluorescence Microscopy and Conjugation. Other applications not tested. Recommended Dilutions: Immunofluorescence: Ice cold acetone fixation of Bacillus spores before IFA staining is highly recommended. Optimal dilutions to be determined by the researcher. Titer: Immunofluorescence: >1:800 Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice