B0003-05J-FITC
Bacillus anthracis, Protective Antigen (Anthrax) (FITC)
Size
£863.00
- SKU:
- B0003-05J-FITC
- Purity:
- Purified by Protein G affinity chromatography (≥95%, SDS-PAGE).
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified protective antigen from inactivated Bacillus anthracis culture (vaccine strain STI-1).
- Clone:
- 2B18
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with Fluorescein isothiocyanate (FITC).
- Extra Details:
- Anthrax toxin plays a major role in the pathogenesis of anthrax. Virulent strains of Bacillus anthracis produce a complex exotoxin with 3 distinct antigenic components: the edema factor (EF), the protective antigen or binding domain (PA), and the lethal factor (LF). Applications: Suitable for use in Western Blot and FLISA. Other applications have not been tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Matched Pair: Use B0003-05H as the capture antibody pair for sandwich FLISA with B0003-05J as the detection antibody. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice