B0003-04
BACH1
Size
£672.00
- SKU:
- B0003-04
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- BACH1/GST fusion protein encompassing the C-terminus of human BACH1.
- Clone:
- 3G11
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
- Extra Details:
- BRCA1 has been shown to interact with a novel member of the DEAH helicase family, BACH12. The BRCA1 gene encodes an 1863 residue nuclear protein with N-terminal RING and C-terminal BRCT domains. BACH1 appears to bind directly to these BRCT repeats of BRCA1. Moreover, BACH1 appears to contribute to the DNA repair of BRCA1. BACH1 derivative bearing a mutation in a key residue that is essential for catalytic function in other helicases interfered with normal DSBR in a manner that was dependent upon its ability to interact with BRCA12. Tumorpredisposing missense and deletion mutations in the BRCA1 BRCT domain, all of which render BRCA1 defective in its DSBR function also disrupted BACH1 binding to BRCA1. BACH1 is an integral part of BRCA1 in DSBR2. Western blots of extracts from three different cell lines revealed a band of 130kD for BACH1 in all MCF7, HeLa and HCC1937 cell line. Immunofluorescence analysis revealed the presence of punctate nuclear staining in multiple human cell lines. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunofluorescence. Other applications not tested. Recommended Dilutions: Western Blot: 1-3ug/ml Immunoprecipitation: Native Optimal dilutions to be determined by the researcher. Positive Controls: MCF7, HeLa and 293 cell lysates. Source: Ascites Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice