B0002-92C

BACE2, Asp1 (Beta-Site APP Cleaving Enzyme 2)

Size

£767.00

SKU:: B0002-92C
Purity:: Affinity purified over the control-peptide Sepharose
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: A 20 aa peptide near the CT of human BACE2/Asp1 (1) conjugated to KLH
Format:: Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 40% glycerol.
Extra Details:: Applications: Suitable for use in Immunofluorescence, ELISA, Western Blotting, Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL. BACE is approx 70kD, greater than the theoretical size of ~51kD, due to glycosylation. ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well. Immunohistochemistry & Immunofluorescence: Not tested. We recommend the use of 2:20ug/ml of affinity pure antibody. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.