B0002-91J

BACE2 (Beta-Site APP Cleaving Enzyme 2, Aspartyl protease 1, Asp1, Memapsin 1, Membrane-anchored aspartic protease of the pepsin family)

Size

£843.00

SKU:: B0002-91J
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Sheep
Immunogen:: Recombinant protein corresponding to Gly63-Pro466 of human BACE2 derived from NS0 cells.
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: BACE-2 (B-site APP cleaving enzyme 2; also ASP-1 and Memapsin-1) is a 73-75kD member of the peptidase A1 family. It is expressed both inside and outside the CNS and is classified as a Golgi aspartyl protease. Its status as a β-secretase is unclear. If it has physiological activity, it may be associated with glia. Mature BACE-2 is a 456aa type I transmembrane glycoprotein. It contains a 411 aa extracellular domain (aa 63-473) that has two catalytic residues (Asp 93 and Asp 289). Two potential splice forms exist; one shows a deletion of aa's 329-378, while a second shows an 18aa substitution for aa380-518. Over aa63-466, human BACE-2 shares 92% aa sequence identity with mouse BACE-2. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: ELISA (Direct): 0.5-1.0ug/ml with the appropriate secondary reagents to detect human and mouse BACE2. The detection limit for BACE-2 is ~0.5ng/well. Western Blot: 0.1-0.2ug/ml with the appropriate secondary reagents to detect human and mouse BACE2. The detection limit for BACE2 is ~5ng/lane and ~25ng/lane under non-reducing and reducing conditions, respectively. Immunoprecipitation: Conditioned media of transfected NS0 cells. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice