B0002-91C

BACE1, Ectodomain (Beta-site APP Cleaving Enzyme)

Size

£863.00

SKU:: B0002-91C
Purity:: Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Thr22-Tyr460 of human BACE1 expressed in NSO cells.
Clone:: 8H105
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: Cleavage of amyloid precursor protein (APP) into 40 or 42 amino acid amyloid beta (ab) peptides is a result of the action of two proteases beta- and gamma-secretase. The beta-site APP cleaving enzyme, or BACE1, is a transmembrane aspartic protease that is localized in the Golgi and endosomes. Low levels of BACE1 mRNA are found in most adult tissues with a higher level of expression in pancreas. Applications: Suitable for use in Immunohistochemistry, Flow Cytometry, Western Blot and Direct ELISA. Other applications not tested. Recommended Dilutions: Immunohistochemistry: 8-25ug/ml detects BACE1 in paraffin-embedded human Alzheimer’s brain tissue sections. Flow Cytometry: 2.5ug/mll labels 10e6 cells in jurkat human acute T cell leukemia cell line fixed with paraformaldehyde and permeabilized with saponin. Western Blot: 2ug/ml detects human brain (Alzheimer's disease hippocampus) tissue Optimal dilutions to be determined by the researcher. Western Blot Buffers: Blotting Buffer: 25mM Tris, pH 7.4, 0.15M sodium chloride, 0.05% Tween-20 Blocking Solution: 5% nonfat dry milk in Blotting Buffer Antibody Solution: 5% nonfat dry milk in Blotting Buffer Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice