B0002-88

BACE, Asp2 (Memapsin2, Beta-site APP Cleaving Enzyme, Aspartyl Protease 2) (Not for Export EU)

Size

£704.00

SKU:: B0002-88
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: 17-aa peptide located near the C-terminus of Human BACE/Asp2 (KLH).
Format:: Supplied as a liquid, neat serum, 40% glycerol.
Extra Details:: Beta-amyloid (Ab) deposition in the brain is the hallmark of Alzheimer's Disease (AD). To initiate Ab formation, b-secretase cleaves APP at the N-terminus of Ab to release APPsb (~100 kD soluble NT-fragment), and C99, a 12-kD CT membrane fragment. Alternatively, a-secretase cleaves within the Ab to prevent the formation of Ab. Cleavage by a-secretase produces a soluble Nterminal fragment, APPsa, and a 10-kD membrane C-terminal fragment, C83. Both C99 and C83 can be further cleaved by gsecretase releasing Ab and a nonpathogenic p3 peptide, respectively. Recently, BACE (Beta-site APP Cleaving Enzyme) has been identified as b-secretase. BACE belongs to the family of Aspartyl proteases (Asp) also known as Memapsins. At least four related Asps, located on chromosome IV and X, have been cloned (Asp1, Asp2, Asp3, and Asp4). Human BACE/Asp2/Memapsin2, located on chromosome 11, is a trans- membrane protein of 501 aa (signal peptide 1-21 aa, a proprotein domain 22-45 aa, 1 TM domain near the CT, and a short cytoplasmic CT- tail of 24 aa; mature protein 46-460 aa). BACE expression was most prominent in most areas of the rat brain and pancreas. It has been localized in the compartments of the secretory pathways. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 ELISA: 1:10,000-50,000. Control peptide can be used to coat ELISA plates at 1ug/ml. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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