A8510-01
ASIALOGLYCOPROTEIN RECEPTOR (ASGPR) (FITC)
Size
£1,093.00
- SKU:
- A8510-01
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Crude rat liver membrane extracts.
- Clone:
- 9G133 (8D7)
- Format:
- Supplied as a liquid in PBS, 1% BSA, 0.02% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
- Extra Details:
- The asialoglycoprotein receptor (ASGPR) is a transmembrane glycoprotein (42kD) which mediates binding, internalisation and degradation of extracellular glycoproteins that have exposed terminal galactose residues. The receptor is expressed on the surface of the hepatocytes in a polar manner, i.e. it is present on the sinusoidal and lateral plasma membranes but not on the bile canalicular membrane. The mammalian hepatic ASGPR mediates the endocytosis and degradation of serum proteins from which terminal sialic residues have been removed. The rat asialoglycoprotein receptor (ASGPR) consists of three polypeptide subunits: rat hepatic lectin (RHL)-1, RHL-2 and RHL-3. Applications: Suitable for use in Flow Cytometry, Immunofluorescence, Immunohistochemistry, FLISA and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 FLISA: 20ug/ml using 100ul/well Western Blot (Non-reduced): 1:50 Immunohistochemistry (frozen, acetone fixed): 1:50 Optimal dilutions to be determined by the researcher. Recommended Positive Control: Rat hepatocytes Recommended Negative Controls: Rat kidney, pancreas, small intestine, colon Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice