A4776-10
Axl, phosphorylated (Tyr779) (AXL Oncogene, AXL Receptor Tyrosine Kinase, AXL Transforming Gene, AXL Transforming Sequence/gene, Adhesion-related Kinase, Ark, Oncogene AXL, Tyrosine Protein Kinase Receptor, UFO, TYR07)
Size
£837.00
- SKU:
- A4776-10
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic phosphopeptide corresponding to human Axl Tyr779 site.
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- Axl (also known as ARK, UFO and TYR07) is a receptor tyrosine kinase that contains extracellular regions composed of two IgG-related domains linked to two fibronectin type III repeats. Gas6, a ligand for Axl, induces Axl phosphorylation on multiple tyrosine residues. Phosphorylation of Axl at Tyr779 is associated with the activation of PI 3-kinase. Applications: Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml detects Axl in Wl38 human lung cell line conditioned media-treated A172 human glioblastoma cell line. Immunocytochemistry: 5-15ug/ml detects Axl in immersion fixed A172 human glioblastoma cell line with and without pervanadate co-staining with total Axl. Immunohistochemistry: 5-15ug/ml detects Axl in immersion fixed paraffin embedded sections of human stomach. Optimal dilution to be determined by researcher. Recommended Secondary Antibodies: I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb I1904-46Q: IgG, H&L (HRP) Pab Gt x Rb Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice