A4747-01
Axin1 (AXIN1, AXIN, Axis Inhibition Protein 1, MGC5231)
Size
£754.00
- SKU:
- A4747-01
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- GST-tagged recombinant protein corresponding to the N-terminus of mouse Axin-1. Species Sequence Homology: mouse
- Clone:
- 10F631 (A5)
- Format:
- Supplied as a liquid in PBS, 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
- Extra Details:
- Axin-1 is constitutively expressed and acts as a negative regulator of Wnt expression. It has been demonstrated that mouse Wnt, Wnt3a, is secreted by multiple cell lines and can act and as a growth factor for stem cells. Secreted Wnt3a is highly hydrophobic because it is palmitoylated on an essential cysteine C77; the palmitoylated state of Wnt3a is essential for stem cell signaling. Incubation of stem cells with Wnt3a in tissue culture medium resulted in the increased number of mouse hematopoietic stem cells expressing "primitive" phenotype. Applications: Suitable for use in Immunoprecipitation, Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Immunoprecipitation (WB): 1ug/ml detected Axin-1 immunoprecipitated from 10ug of SW-480 cell lystate. Optimal dilutions to be determined by the researcher. Positive Control: SW-480 cell lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice