A4190

Aurora A (Serine/Threonine-protein Kinase 6, Aurora Kinase A, Aurora/IPL1-related Kinase 1, Aurora-related Kinase 1, ARK-1, hARK1, Breast Tumor-amplified Kinase, AURKA, ARK1, AURA, BTAK, STK6, AIK)

Size

£581.00

SKU:: A4190
Purity:: Purified by Protein A and Peptide affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to the N-terminus of human Aurora A/AIK.
Format:: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Extra Details:: Aurora A/AIK, a cell cycle-regulated serine/threonine protein kinase, is overexpressed in many tumor cell lines. Phosphorylation of threonine 288, which is within the activation loop of the kinase, results in a significant increase in its enzymatic activity. Aurora A/AIK is regulated by proteasome-dependent degradation, and phosphorylation on Thr288 may target it for degradation during mitosis. Thus, phosphorylation of Aurora A/AIK at Thr288 is important for both kinase activity and stability. Applications: Suitable for use in ELISA, and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1,000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.