A4190-17C-ML650
Aurora C (Serine/threonine-protein kinase 13, Serine/threonine kinase AIE2, Aurora IPL1/Eg2 protein 2, Aurora IPL1-related kinase 3) (MaxLight 650)
Size
£1,042.00
- SKU:
- A4190-17C-ML650
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide derived from an internal region of the human Aurora C (Serine/threonine-protein kinase 13, Serine/threonine kinase AIE2, Aurora/IPL1/Eg2 protein 2, Aurora/IPL1-related kinase 3) protein.
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Aurora C1 is a member of the aurora/Ipl1p family of protein kinases that are important regulators of mitosis.2 Aurora C is a novel chromosomal passenger protein that, in coordination with Aurora B, regulates mitotic chromosome dynamics in human cells.3,4 Aurora C may play a role in organizing microtubules relating to the function of the centrosome/spindle pole during mitosis, and is localized to the centrosome from anaphase to cytokinesis.5 The expression of Aurora C is maximum at M phase.5 Aurora C is activated by directly associating with inner centromere protein (INCENP).3 Aurora C is expressed in normal testis6, and elevated expression levels are seen in a subset of cancer cells such as HepG2 and HeLa cells.5 Applications: Suitable for use in Western Blot. Other applications have not been tested. Recommended Dilution: Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Human placenta homogenates, and human JEG3, HEPG2, HeLa, HEK293, NGP96, SKNEP-1 and U251 cell lysates. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice