A4189-21

Aurora B (STK12, Serine Threonine Kinase 12, Aurora Kinase B, AIK2, AIM1, AurB, IPL1, ARK2, Aurora Related Kinase 2)

Size

£914.00

SKU:: A4189-21
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to aa225-250 from the internal region of human Aurora-B kinase. Species sequence homology: rat, bovine, mouse, chimpanzee, canine and porcine.
Format:: Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.
Extra Details:: Aurora-B kinase (AurB) is a serine/threonine kinase which is the enzymatic core of the Chromosomal Passenger Complex (CPC). This complex functions in chromosome alignment and separation, histone modification, and cytokinesis. CPC also includes three non-enzymatic subunits known as the inner centromere protein (INCENP), survivin, and borealin, which determine the activity, localization, stability, and possibly even the substrate specificity of AurB. Two CPCs exist during mitosis - one containing all four members, and another consisting of INCENP and AurB. Quaternary CPC functions during chromosome alignment and cytokinesis, whereas the INCENP-AurB complex may be responsible for modifying histone H3. AurB is overexpressed in a number of tumors, including primary breast and colon tumor samples. Its expression in tumors seems to be linked with Aurora-A kinase, suggesting a feedback mechanism between the two proteins. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 1:5000-1:20,000 Western Blot: 1:50-1:2000, detects a band of ~39kD in HeLa cell lysates Optimal dilutions to be determined by the researcher. Positive Control: Human brain tissue lysate or HeLa cell lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice