A4189-11
Aurora B Serine/Threonine Kinase, Recombinant, Human full length (AURKB, STK12, Serine Threonine Kinase 12, Aurora B, AIK2, AIM1, AurB, IPL1, ARK2, Aurora Related Kinase 2)
Size
£912.00
- SKU:
- A4189-11
- Storage Conditions:
- -70°C
- Supplier:
- United States Biological
- Format:
- 25 mM Tris-HCl, pH 8.0, 100 mM sodium chloride, 0.05% Tween 20, 50% glycerol, 3mM DTT.
- Extra Details:
- Human Aurora kinase B (GenBank Accession No. NM_004217), (a.a. 2-344), expressed in an Baculovirus infected Sf9 cell expression system. Stability: 6 months at –80 °C Purity: >95% by SDS-PAGE. Specific Activity: 22 U/ug. One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Ser/Thr 1 peptide substarte per minute at pH 7.4 and 30 °C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3uM Na orthovanadate, 0.5 mM ATP, 4 uM aurora B substrate, and 0.2 ug Aurora B kinase. Application: useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
- Shipping Conditions:
- Dry Ice