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A4000-72B1

ATPase beta 1, Na, K

Size

£825.00

SKU:
A4000-72B1
Purity:
Purified by Protein A affinity chromatography.
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Mouse
Immunogen:
Purified sheep kidney Na+/K+ ATPase-beta.
Clone:
6D136
Format:
Supplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide.
Extra Details:
The Na,K-ATPase is an integral membrane protein complex responsible for establishing the electrochemical gradients of Na+ and K+ ions across the plasma membranes of mammalian cells. The enzyme consists of an a and b subunit, both of which exist as isoforms. The a subunit is believed to be the catalytically active subunit, while the b subunit appears necessary for the assembly and transport of the sodium pump to the plasma membrane. The beta subunit is a membrane spanning glycoprotein present in all cell types. The antigenic determinant of this antibody has been mapped to the ectodomain (~residues 62-304) of the beta subunit. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry and Immunofluorescence. Other applications not tested. Recommended Dilutions: Immunohistochemistry: 1:100 Immunohistochemistry (Paraffin): 1:200 Immunofluorescence: 1:100-1:1000 Western Blot: 1:1000-1:10,000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Shipping Conditions:
Blue Ice