A4000-70
ATPase beta 1, Na, K
Size
£1,010.00
- SKU:
- A4000-70
- Purity:
- Purified by Protein G affinity chromatography from ascites.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified Na,K-ATPase isolated from membrane fractions of rabbit kidney outer medulla.
- Clone:
- 0.T.05 (C464.8)
- Format:
- Supplied as liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
- Extra Details:
- The Na,K-ATPase is an integral membrane protein complex responsible for establishing the electrochemical gradients of Na+ and K+ ions across the plasma membranes of mammalian cells. The enzyme consists of an a and b subunit, both of which exist as isoforms. The a subunit is believed to be the catalytically active subunit, while the b subunit appears necessary for the assembly and transport of the sodium pump to the plasma membrane. The beta subunit is a membrane spanning glycoprotein present in all cell types. The antigenic determinant of this antibody has been mapped to the ectodomain (approximate residues 62-304) of the beta subunit. Applications: Suitable for use in Western Blot and Immunohistochemistry, Other applications not tested. Recommended Dilutions: Western Blot: 0.5-2ug/ml detects ATPase beta 1, Na, K in 20ug of rat brain microsomal preparation. A previous lot detected ATPase beta 1, Na, K using rat heart and rat kidney microsomal preparations. Rat brain microsomal preparation was resolved by electrophoresis, transferred to PVDF. Proteins were visualized using an IgG (HRP) goat anti-mouse secondary antibody and a chemiluminescence detection system. Immunohistochemistry: 10ug/ml on 4% paraformaldehyde fixed tissue. Optimal dilutions to be determined by the researcher. Hybridoma: Ag8 mouse myeloma cells with immunized Balb/c splenocytes. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice