A4000-70-APC
ATPase beta 1, Na, K (APC)
Size
£1,246.00
- SKU:
- A4000-70-APC
- Purity:
- Purified by Protein G affinity chromatography from ascites.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified Na,K-ATPase isolated from membrane fractions of rabbit kidney outer medulla.
- Clone:
- 0.T.05 (C464.8)
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
- Extra Details:
- The Na,K-ATPase is an integral membrane protein complex responsible for establishing the electrochemical gradients of Na+ and K+ ions across the plasma membranes of mammalian cells. The enzyme consists of an a and b subunit, both of which exist as isoforms. The a subunit is believed to be the catalytically active subunit, while the b subunit appears necessary for the assembly and transport of the sodium pump to the plasma membrane. The beta subunit is a membrane spanning glycoprotein present in all cell types. The antigenic determinant of this antibody has been mapped to the ectodomain (approximate residues 62-304) of the beta subunit. Applications: Suitable for use in Western Blot and Immunohistochemistry, Other applications not tested. Recommended Dilution: Western Blot: detects ATPase beta 1, Na, K in 20ug of rat brain microsomal preparation. A previous lot detected ATPase beta 1, Na, K using rat heart and rat kidney microsomal preparations. Rat brain microsomal preparation was resolved by electrophoresis, transferred to PVDF. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunohistochemistry: 4% paraformaldehyde fixed tissue. Optimal dilutions to be determined by the researcher. Hybridoma: Ag8 mouse myeloma cells with immunized Balb/c splenocytes. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice