A4000-50A5
PMCA1 (PMCA ATPase)
Size
£800.00
- SKU:
- A4000-50A5
- Purity:
- Ascites
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified human erythrocyte calcium ATPase.
- Clone:
- 5F10
- Format:
- Supplied as a liquid in PBS, 0.05% sodium azide.
- Extra Details:
- Ca2+ plays a critical role in intracellular signaling. Intracellular Ca2+ levels are tightly controlled by continuous removal of Ca2+ via ATP-driven Ca2+ pump in the endoplasmic reticulum and plasma membrane, and Ca2+ transport system, the Na+/Ca2+ exchangers (NCX) in the plasma membrane. NCX can move Ca2+ either into or out of cells, depending on the net Na+, Ca2+ and K+ gradient across the membrane. In most cells, 3 Na+ are exchanged for 1 Ca2+. In mammals, at least 5 distinct genes code for the exchangers: Three NCX (NCX1, NCX2, and NCX3), and two in the NCKX family (NCKX1 and NCKX2). Applications: Suitable for use in Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications have not been tested. Recommended Dilution: Immunohistochemistry (Frozen, paraffin): 1:500 Western Blot: 1:1000. Detects a band of ~140kD. Also detects bands at ~95kD and ~180kD which may represent aggregation and/or natural proteolytic products of PMCA ATPase. Optimal dilution to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice