A4000-50A3

ATPase, Ca (Plasma Membrane, PMCA) (Not for Export EU)

Size

£704.00

SKU:: A4000-50A3
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: A 20 AA Peptide sequence near the N-terminus of mature Rat NCKX2 (1). The peptide was coupled to KLH
Format:: Supplied as a liquid, neat serum, 40% glycerol.
Extra Details:: Ca2+ plays a critical role in intracellular signaling. Intracellular Ca2+ levels are tightly controlled by continuos removal of Ca2+ via ATP-driven Ca2+ pump in the endoplasmic reticulum and plasma membrane, and Ca2+ transport system, the Na+/Ca2+ exchangers (NCX), in the plasma membrane. NCX can move Ca2+ either into or out of cells, depending on the net Na+, Ca2+, and K+ gradient across the membrane. In most cells, 3 Na+ are exchanged for 1 Ca2+. In mammals, at least 5 distinct genes code for the exchangers: Three NCX (NCX1, NCX2, and NCX3), and two in the NCKX family (NCKX1 and NCKX2). NCX share significant sequence homology (~70%), display 11 TM domains, a large central, intracellular hydrophilic regulatory loop between TM5 and 6, extracellular N-terminus and cytoplasmic C-terminus. The N-terminal signal peptide is cleaved off from the mature exchanger protein. NCX contains a highly basic region in the large hydrophilic, intracellular loop called XIP (Exchange inhibitory peptide; RRLLFYKYVYKRYRAGKQRG 20 aa), that inhibits Na-Ca+ exchange in cardiac sarcolemmal vesicles and in other cells. Little or no sequence identity is found between the NCX and the Ca-pump. However, XIP also inhibits the Ca pumps with more or less same efficiency as C28R2 peptide sequence (LRRGQILWFRGLNRIQTQIRVVKAFRSS, 28 aa) corresponding to the autoinhibitory domain of Ca-pump. Ca+-pump is a Mg+-dependent enzyme that catalyzes the hydrolysis of ATP with the transport of Ca+. Plasma membrane Ca+-ATPase (calcium pump) exists in several isoforms (human isofroms 1, 1084 aa; 1b, 1220 aa; from 2, 198/1243 aa; 3a/b 1173/1220 aa; 4, 1205 aa) and numerous sub-isoforms have been identified in various species. Ca+-pump displays 10 TM domains, with cytoplasmic N and C-termini. C28R2 is located in within the cytoplasmic, C-terminal domain of Ca+-ATPases. Applications: Suitable for use in ELISA, Western Blotting. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using ECL. ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity purified. Control peptide can be used to coat ELISA plates at 1ug/ml. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice