A4000-19E
ATM (Serine-protein Kinase ATM, Ataxia Telangiectasia Mutated, A-T Mutated) [KO Validated]
Size
£832.00
- SKU:
- A4000-19E
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to aa2577-3056 from human ATM expressed in E. coli.
- Clone:
- 2C1
- Format:
- Supplied as a liquid in PBS, pH 7.4. No preservative added.
- Extra Details:
- ATM belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2,checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. ATM and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in the gene encoding ATM are associated with ataxia telangiectasia, an autosomal recessive disorder. Applications: Suitable for use in Flow Cytometry, ELISA, Immunofluorescence, Western Blot, Immunoprecipitation, Immunocytochemistry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (paraffin): 5ug/ml Western Blot: 1:500-1:3000 Immunoprecipitation: 1-10ug/ml Immunofluorescence (IC): 1:10-1:500 ELISA: 1:100-1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice