A3999-82B
ATP7b (PWD, WC1, WND)
Size
£594.00
- SKU:
- A3999-82B
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide made to an internal sequence nearer the C-terminus of human ATP7b
- Format:
- Supplied as a liquid containing 0.09% sodium azide
- Extra Details:
- ATP7b is an important protein for copper transport and elimination of excess copper from the body. ATP7b transports metals in and out of cells using ATP. There are 3 known isoforms of the ATP7b gene; A is found in the liver, kidney, and brain, the shorter form B is found in brain tissue, and the third isoform, known as WND/140kD is found in mitochondria. Mutations in the ATP7b gene can cause Wilson’s disease, an inherited disorder causing copper poisoning in the brain and liver. Applications: Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000. Recognizes a band at 165kD, representing ATP7b protein. Also recognizes a band at ~220kD. Immunocytochemistry: 1:500 using ovarian carcinoma cells Optimal dilutions to be determined by the researcher. Positive Control: HuH7 hepatocarcinoma cell extracts Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice