A3999-82A
ATP7b (PWD, WC1, WND)
Size
£594.00
- SKU:
- A3999-82A
- Purity:
- Purified by affinity chromatography
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide made to an internal sequence near the N-terminus of human ATP7b
- Format:
- Supplied as a liquid in Tris citrate/phosphate buffer, pH 7.5, 0.09% sodium azide.
- Extra Details:
- ATP7b is an important protein for copper transport and elimination of excess copper from the body. ATP7b transports metals in and out of cells using ATP. There are 3 known isoforms of the ATP7b gene; A is found in the liver, kidney, and brain, the shorter form B is found in brain tissue, and the third isoform, known as WND/140kD is found in mitochondria. Mutations in the ATP7b gene can cause Wilson’s disease, an inherited disorder causing copper poisoning in the brain and liver. Applications: Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (Paraffin): 1:500 Western Blot: 1:1000. Band at ~165kD Immunocytochemistry: 1:500 Optimal dilutions to be determined by the researcher. Positive Control: HuH7 hepatocarcinoma cell extracts Cellular Localization: Golgi apparatus; Predominantly found in trans-Golgi network membrane; multi-pass membrane protein (By similarity). Isoform 2: Cytoplasm. The cleaved form WND/140kD is located in mitochondria. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice