A3950-01-BIOTIN
ATH 1 (MATH 1, NeuroD Family, Helix-loop-helix Transcription Factor) (Biotin)
Size
£1,119.00
- SKU:
- A3950-01-BIOTIN
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to aa188-200, SFNNDKKLSKYET of human ATH1.
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
- Extra Details:
- ATH 1 (MATH 1, NeuroD Family) belongs to the basic helix-loop-helix (BHLH) family of transcription factors. It activates E-box dependent transcription. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Recommended Control Peptide: A3950-01A IMMUNOHISTOCHEMISTRY Protocol: 1. Dissect tissues and freeze on dry ice. 2. Cut on a cryostat -10um slices. 3. Dry slides at RT for 30-90 min. 4. Fix with cold Acetone/Methanol (50/50) for 2 min. Either process further or store at -20°C. Alternative is fixation with 4% PFA and treatment with a trypsin solution. (0.05% trypsin, incubation for 10 minutes at 37°C, followed by 3 washes with PBS). 5. Let air dry. 6. Treat with PBS for 5 min. 7. Incubate in 50mM ammonium chloride for 30 min. 8. Treat with PBS for 5 min. 9. Treat with Blocking serum for 30-45 min. 10. Treat with A3950-01 for 90 min. 11. Wash 3 times for 5 min. 12. Secondary fluorescent conjugate antibody for 30 min. 13. Wash 3 times for 5 min and mount. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice