A3886
ADMA (Asymmetric Dimethylarginine)
Size
£433.00
- SKU:
- A3886
- Purity:
- Purified by Protein G affinity chromatography from serum.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- ADMA, conjugated to KLH.
- Format:
- Supplied as a lyophilized powder from 50mM Tris, pH 7.4. Reconstitute with 100ul sterile ddH2O.
- Extra Details:
- Asymmetric dimethylarginine (ADMA) is a naturally occurring chemical found in blood plasma. It is a metabolic by-product of continual protein modification processes in the cytoplasm of all human cells. It is closely related to L-arginine, a conditionally-essential amino acid. ADMA interferes with L-arginine in the production of nitric oxide, a key chemical involved in normal endothelial function and, by extension, cardiovascular health. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Paraffin sections Optimal dilutions to be determined by the researcher. Crossreactivity: Using conjugated ADMA-KLH, antibody specificity was performed with an ELISA test by competition experiments with the following compounds: Compound: Cross-reactivity ratio(a) ADMA-BSA: 1 SDMA-BSA: 1:>100 NDMA-BSA: 1:>100 L-Arginine-BSA: 1:>5000 (a) ADMA-BSA concentration/unconjugated or conjugated amino acids concentration at half displacement BSA=Bovine Serum Albumin Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice