ASPM (ASP (Abnormal Spindle)-like Microcephaly Associated, Microcephaly, Primary Autosomal Recessive 5, HGNC:19048, MCPH5) (Biotin)

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£927.00

SKU:: A3883-40A-BIOTIN
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Linear peptide corresponding to human ASPM conjugated to KLH. Epitope: Internal. Species Sequence Homology: chimpanzee and primate
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Extra Details:: ASPM gene is the human ortholog of the Drosophila melanogaster 'abnormal spindle' gene (asp), which is essential for normal mitotic spindle function in embryonic neuroblasts (Bond et al., 2002 [PubMed 12355089]). The mouse gene ASPM is expressed specifically in the primary sites of prenatal cerebral cortical neurogenesis. Molecular Weight: 218, 95, 75kD Observed UniProtKB/Swiss-Prot entry Q8IZT6 describes 2 isoforms produced by alternative splicing: Isoform 1 at 409.800kD and Isoform 2 at 217.794kD. Multiple other isoforms in ref: Human Molecular Genetics, 2005, Vol. 14, No. 15 2155-2165. Applications: Suitable for use in ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: Tested in direct ELISA against the ASPM immunogen. Immunohistochemistry (Paraffin): 1:300. Positive and background staining patterns on formalin fixed, paraffin embedded human ductal carcinoma. Tissue pretreated with citrate pH 6.0 using antigen retrieval techniques. Detected using HRP/DAB. Immunoreactivity is seen in nucleus and cytoplasm. Optimal dilutions to be determined by the researcher. Positive Control: Human ductal carcinoma tissue Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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