ASPM (ASP (Abnormal Spindle)-like Microcephaly Associated, Microcephaly, Primary Autosomal Recessive 5, HGNC:19048, MCPH5) (APC)

Size

£927.00

SKU:: A3883-40A-APC
Purity:: Purified by affinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Linear peptide corresponding to human ASPM conjugated to KLH. Epitope: Internal. Species Sequence Homology: chimpanzee and primate
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Extra Details:: ASPM gene is the human ortholog of the Drosophila melanogaster 'abnormal spindle' gene (asp), which is essential for normal mitotic spindle function in embryonic neuroblasts (Bond et al., 2002 [PubMed 12355089]). The mouse gene ASPM is expressed specifically in the primary sites of prenatal cerebral cortical neurogenesis. Molecular Weight: 218, 95, 75kD Observed UniProtKB/Swiss-Prot entry Q8IZT6 describes 2 isoforms produced by alternative splicing: Isoform 1 at 409.800kD and Isoform 2 at 217.794kD. Multiple other isoforms in ref: Human Molecular Genetics, 2005, Vol. 14, No. 15 2155-2165. Applications: Suitable for use in FLISA and Immunohistochemistry. Other applications not tested. Recommended Dilutions: FLISA: Tested in direct FLISA against the ASPM immunogen. Immunohistochemistry (Paraffin): 1:300. Positive and background staining patterns on formalin fixed, paraffin embedded human ductal carcinoma. Tissue pretreated with citrate pH 6.0 using antigen retrieval techniques. Detected using HRP/DAB. Immunoreactivity is seen in nucleus and cytoplasm. Optimal dilutions to be determined by the researcher. Positive Control: Human ductal carcinoma tissue Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

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